Tn-seq of RPE75 growth in minimum media with glucose and 1.56 g.mL-1 ... - SRA

SRX17888461: Tn-seq of RPE75 growth in minimum media with glucose and 1.56 g.mL-1 of polymyxin B (Control sample)
1 ILLUMINA (NextSeq 500) run: 2.9M spots, 48.4M bases, 8.5Mb downloads

Design: HIMAR transposon insertion sites amplified by PCR from genomic DNA from a transposon mutant library; genomic DNA was digested with MmeI and adaptors were ligated before PCR amplification

Submitted by: Centre National de la Recherche Scientifique

Study: Identification of antimicrobial peptide resistance genes in Caballeronia Insecticola

PRJNA890438 • SRP402594 • All experiments • All runs

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We determined the genetic repertoire in Caballeronia insecticola to manage antimicrobial peptide (AMP) stress. Transposon sequencing experiments were performed in the presence of the generic AMP polymyxin B (PMB).

Sample: Tn-seq of RPE75 growth in minimum media glucose with 1.56 g.mL-1 of polymyxin B

SAMN31278432 • SRS15405661 • All experiments • All runs

Organism: Caballeronia insecticola

Library:

Name: PMB_1.56_R1

Instrument: NextSeq 500

Strategy: Tn-Seq

Source: GENOMIC

Selection: PCR

Layout: SINGLE

Runs: 1 run, 2.9M spots, 48.4M bases, 8.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR21902401	2,929,252	48.4M	8.5Mb	2022-10-14

ID:: 24822069

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